100 mM Sodium orthovanadate

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

100 mM Sodium orthovanadate

Requirements

1.839 g Sodium orthovanadate 8ml distilled water to 8 ml

Method

  • Add 1.839 g Sodium orthovanadate to a screw cap tube (falcon)
  • Make up to 8ml with distilled water.
  • Adjust pH to 10
  • If solution is yellow, place in boiling water until clear. Re-check pH and repeat as necessary.
  • Adjust to final concentration by checking A265 nm, ext. coeff.= 2925M-1cm-1and adding distilled water as needed. Store until required.

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00