10x Tris BufferΒΆ
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
Recipe for 10x Tris Buffer, dilute for use
- Fill suitable container with 1L dH20
- Add 30g Tris, 88g NaCl and 2g KCl to the solution, mixing thoroughly until fully dissolved.
- Adjust pH to 7.5 with HCl