10x Tris BufferΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Recipe for 10x Tris Buffer, dilute for use

  • Fill suitable container with 1L dH20
  • Add 30g Tris, 88g NaCl and 2g KCl to the solution, mixing thoroughly until fully dissolved.
  • Adjust pH to 7.5 with HCl