150 mM Mg AMP-PNP

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

150 mM Mg AMP-PNP

Requirements

25 mg AMP-PNP (available from Boehringer Mannheim #102 547) 90 ul 1 M MgSO4 Stock (150 mM MgSO4) 225 ul distilled water

Method

  • Add 25 mg AMP-PNP and 90ul 1 M MgSO4 in a suitable container.
  • Add 225 ul distilled water to give a final volume of 315 ul. Store at -20oC

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00