1M dithiothreitol (DTT)

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

1M dithiothreitol (DTT)

Requirements

1.542 g dithiothreitol 10ml Distilled water

Method

  • Add dithiothreitol to a suitable container. Make up to 10ml with distilled water.
  • Aliquot in 500 ml volumes and store at -20oC for future use.

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00