2X Rapid Ligation Buffer

Contributed by Ian Chin-Sang, Queens University, ON, Canada

2X Rapid Ligation Buffer (ProMega)

Requirements

60mM Tris-HCl (pH 7.8) 20mM MgCl2 20mM DTT 2mM ATP 10% polyethylene glycol

Method

• Combine ingredients in suitable container.

This method is based, with permission, on an original protocol available here.