Silane/acetone silanized slides¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
Silanized (3-aminopropyltriethoxysilane) slides to prevent sections from detaching.
- Place slides in 2% silane/acetone solution for 1 minute
- Transfer slides to 100% acetone for 1 minute
- Transfer slides to double distilled water. Agitate for 1 minute
- Replace double distilled water, and wash repeat x2 for 1 minute
- Leave slides to air dry overnight at room temperature
- Slides may be kept indefinitely covered in slide box until ready for use