Chromosome FISHΒΆ
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
DAPI counterstain for chromosome FISH analysis
- After FISH sample preparation briefly rinse sample in distilled water to remove residual salts
- Dilute stock DAPI to a concentration of 30nM in PBS
- Add 300ul of diluted DAPI solution directly onto the sample, ensuring sample is covered equally
- Incubate at room temperature for 30 minutes in the dark
- Rinse specimen with distilled water or PBS to remove DAPI solution
- Blot edge of slide with tissue to remove excess water/solution from the side
- Mount slide with appropriate slide mountant, nail polish or wax.